Breast cancer remains one of the leading causes of cancer mortality among women, and the study of epigenetic mechanisms is an important task of molecular oncology in breast cancer. In this study, we analyzed the expression levels of 8 microRNAs (miR-125b-5p, -127-5p, -129-5p, -132-3p, -148a-3p, -193a-5p, -24-2-5p, -34b-3p) and methylation of promoter regions of 7 microRNA genes in a representative sample of 40 and 70 paired samples of tumor and normal breast tissue, respectively, and showed hypermethylation of promoter regions of 7 genes and statistically significant decrease in expression levels of 8 microRNAs in tumor. For three genes (MIR125B-1, MIR129-2, MIR148A), inverse relationships between methylation and expression (r −0.5) were revealed, indicating their possible epigenetic regulation. Statistically significant positive correlations of expression levels were revealed for 7 pairwise combinations of miRNAs, suggesting their coordinated functioning. Indeed, for the pairs miR-127-5p/miR-125b-5p, miR-148a-3p/miR-125b-5p, miR-148a-3p/miR-132-3p, miR-34b-3p/miR-193a-5p, common mRNA targets and involvement in biological processes, including pathways associated with epigenetic regulation, proliferation and metastasis, were revealed. The miRNA–mRNA regulatory network constructed involving DNMTs and EZH2 highlights their potential role in breast cancer progression and demonstrates diagnostic and prognostic significance.
E.A. Filippova (Wed,) studied this question.