What is the clinical evidence from this study?

Study design: Observational. Population: Insulin resistance (n=17). Intervention: Thigh subcutaneous adipose tissue vs. Abdominal subcutaneous adipose tissue. Primary outcome: GATA3 mRNA expression in insulin-resistant individuals.

January 7, 2026Open Access

Abdominal versus femoral subcutaneous fat gene expression in non-obese insulin resistant individuals

Key Result

In non-obese insulin-resistant individuals, GATA3 mRNA levels were twice as high and PPAR-γ levels were 1.5 times higher in thigh subcutaneous adipose tissue compared to abdominal tissue.

Study Design

Type

Observational (n=17)

Multicenter

Structured PICO

Population

17 non-obese individuals (lean and overweight) scheduled for body contouring surgery, categorized into insulin-sensitive (n=10) and insulin-resistant (n=7) groups based on HOMA-IR. Excluded: BMI ≥30 kg/m², unstable weight in the past six months, diabetic or on diabetes medication, or had undergone obesity surgery within the previous two years.

Outcome

Adipose depot-specific differences in gene expression (GATA3, PPAR-γ, adiponectin, PGC-1α, TNF-α, IL-6) in abdominal versus femoral subcutaneous adipose tissuesurrogate

Functional differences between abdominal and thigh subcutaneous adipose tissue involve depot-specific transcriptional regulation of adipogenic factors like GATA3, which is altered in insulin resistance.

Limitations

Relatively small sample size limits generalizability
Focuses solely on non-obese individuals, which may not capture the full spectrum of metabolic variations in obese populations
Unable to evaluate PPAR-γ protein expression due to technical challenges
Cross-sectional design does not establish causality between vitamin D levels and gene expression

Abstract

Introduction: Adipose tissue, once viewed primarily as an energy reservoir, is now recognized as an endocrine organ involved in various physiological functions, including energy balance and immune responses. Interestingly, recent insights suggest that not all overweight or obese individuals exhibit insulin resistance, guiding the focus towards depot-specific adipose gene expression and its metabolic implications. This pilot study investigates adipose depot-specific differences in gene expression in abdominal versus femoral subcutaneous adipose tissue (SAT) among non-obese, insulin-sensitive (IS) and insulin-resistant (IR) individuals, aiming to elucidate underlying mechanisms of metabolic dysfunction. Methods: Seventeen non-obese participants scheduled for body contouring surgery provided paired SAT biopsies from abdominal and femoral regions. Based on HOMA-IR scores, participants were categorized into IS and IR groups. Gene expression of key markers including GATA3, PPAR-γ, adiponectin, PGC-1α, TNF-α, and IL-6 were analyzed using real-time PCR and Western Blot. Vitamin D levels were also measured. Results: Our analysis revealed a complex depot-specific regulation of GATA3 expression in human subcutaneous adipose tissue. While GATA3 protein showed a trend toward higher expression in abdominal SAT, its mRNA levels were significantly elevated in thigh SAT exclusively in insulin-resistant individuals. Furthermore, circulating vitamin D levels correlated significantly with GATA3 and PPARγ expression specifically in thigh adipose tissue. Conclusion: This pilot study reveals new evidence that the functional differences between abdominal and thigh subcutaneous adipose tissue also encompass transcriptional and post-transcriptional regulation of key adipogenic factors.

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