What question did this study set out to answer?

The research aims to improve the purification methods for extracellular vesicles and investigate their internalization into trypanosomatid cells.

February 2, 2026Open Access

Optimization of Extracellular Vesicle Purification Using a Fluorescent Dye for Visualizing Their Internalization into Trypanosomatid Cells

Key Points

The research aims to improve the purification methods for extracellular vesicles and investigate their internalization into trypanosomatid cells.
Utilized a fluorescent dye to enhance visualization of extracellular vesicles.
Conducted experiments on trypanosomatid cells to assess the internalization of these vesicles.
Investigated optimization techniques for extracellular vesicle purification.
Demonstrated successful internalization of extracellular vesicles into trypanosomatid cells.
Optimized purification methods led to higher yields of identifiable extracellular vesicles.
Visualization with the fluorescent dye improved detection of vesicle uptake.

Abstract

Conference & School on Extracellular Vesicles and Nanoparticles (CSEVP-2024) (Tor Vergata University, Rome, Italy, December 2-4, 2024) This research was made possible thanks to funding through the MSCA4Ukraine project 1232897 "Study of the anti-leishmanial activity of nanoparticles considering their effect on the microbiome", which is funded by the European Union. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union. Neither the European Union nor the MSCA4Ukraine Consortium as a whole nor any individual member institutions of the MSCA4Ukraine Consortium can be held responsible for them (https://sareurope.eu/ msca4ukraine).

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