What question did this study set out to answer?

The aim is to evaluate how an engineered enzyme can inhibit biofilm formation by disrupting bacterial signaling.

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February 28, 2026

Microscopic Assessment of Enzyme-Mediated Inhibition of Biofilm Formation

Key Points

The aim is to evaluate how an engineered enzyme can inhibit biofilm formation by disrupting bacterial signaling.
Introduced an enzyme that disrupts bacterial cell signaling.
Assessed biofilm development on a glass-bottomed microdish.
Visualized biofilm structure using fluorescent lectins and confocal laser scanning microscopy.
The enzyme significantly reduced the production of extracellular polymeric substances.
Biofilm formation was visually decreased as evidenced by microscopy.

Abstract

Source: Tay, S. B., et.al. Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases. J. Vis. Exp. (2016)This video demonstrates the process of inhibiting biofilm formation by introducing an enzyme that disrupts bacterial cell signaling. The enzyme prevents the bacteria from producing extracellular polymeric substances, thereby reducing biofilm development on a glass-bottomed microdish. Finally, the biofilm structure is visualized using fluorescent lectins and confocal laser scanning microscopy.

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TODO TODO (Thu,) studied this question.

synapsesocial.com/papers/69a286490a974eb0d3c0117a https://doi.org/https://doi.org/10.3791/24428