What question did this study set out to answer?

The aim is to extract total DNA from fish tissues to enable further genetic analyses.

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March 1, 2026

SOP-006 DNA extractions from fish tissue

Key Points

The aim is to extract total DNA from fish tissues to enable further genetic analyses.
Extracted DNA from cod and hake tissues including fin and liver.
Lysed samples using proteinase K followed by RNase treatment.
Used a spin column for DNA binding during centrifugation.
Washed to remove contaminants and inhibitors before DNA elution.
Successfully extracted DNA from fin and liver tissues of cod and hake.
Elimination of RNA was confirmed through rinse steps.
DNA is ready for downstream applications after elution.

Abstract

Extraction of total DNA from cod and hake tissues (e.g. fin and liver) in order to be used for downstream analyses. Cited modified from Qiagen Handbook DNeasy Blood & Tissue Kit: “Samples are first lysed using proteinase K. The lysate is subsequently treated with RNase to remove RNA (even fin tissue contains high amounts of RNA) and loaded onto a spin column. During centrifugation, DNA is selectively bound while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed with wash steps and DNA is then eluted in water or buffer, ready for use.”

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