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This DNA extraction protocol was used in Rodriguez et al., 2024 (in prep) for the extraction of environmental DNA from samples collected off of the coast of Ireland (Atlantic) and Norway (Norwegian Sea) as a part of the eWHALE project. Work was carried out by researchers at University College Cork (UCC) and the Institute for Marine Research (IMR). The protocol outlines lysis from Sterivex (0.45 µm pore size; Merck Millipore ID: SVHV010RS) eDNA filters and DNA extraction of lysates using the Qiagen DNeasy Blood and Tissue Kit. Mostly, the protocol follows the official version with spin columns published by Qiagen for the DNeasy Blood and Tissue Kit (available here: https://www.qiagen.com/us/resources/resourcedetail?id=68f29296-5a9f-40fa-8b3d-1c148d0b3030&lang=en), however, researchers at IMR used a QiaVAC 24 Plus vacuum system (ID: 19413, Qiagen) rather than centrifugation for spin column steps.
Rodriguez et al. (Fri,) studied this question.
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