Rapid Mycoplasma Detection Techniques for Facilitating Quality in Low-Volume T-Cell Therapies

Puntos clave

• The study aims to evaluate rapid detection techniques for mycoplasma in low-volume T-cell therapies to ensure quality control.
• Processed samples using Applied Biosystems™ PrepSEQ™ manual and automated workflows.
• Performed alcohol precipitation on samples for purification.
• Conducted PCR using the Applied Biosystems™ QuantStudio™ 5 Real-time PCR system.
• Enhanced detection rates of mycoplasma using both workflows.
• Automated techniques showed improvements in processing time.
• Real-time PCR provided reliable and quantifiable results.