Mechanism of italicAnwei Decoction/italic （安胃汤） in inducing cell cycle arrest in MC cells

Key Points

• The research aims to explore the mechanism by which Anwei Decoction induces cell cycle arrest in MC cells.
• Compared GES-1 (control), MC (model), gastric recovery, and Anwei Decoction groups.
• Assessed cell viability using CCK8 at multiple time points.
• Analyzed cell cycle phases using flow cytometry.
• Evaluated DNA damage through DNALADDER.
• Monitored protein expression via Western blot for relevant markers.
• Anwei Decoction serum at 20% significantly reduced MC cell viability to 52.3% after 36 hours (<P<0.0001>).
• Induced G1/S phase arrest in MC cells with increased G1 and decreased S phase (<P<0.0001>).
• Increased DNA damage observed in the Anwei Decoction group compared to others.
• Changes in protein expression indicated decreased HSP90 and Alpha, and elevated CyclinE1 in Anwei Decoction treated cells.

Abstract