What question did this study set out to answer?

To evaluate the aerobic denitrification capability of Stutzerimonas stutzeri KA1 for improving nitrogen removal from coking wastewater.

March 8, 2026Open Access

Isolation of aerobic denitrifying bacteria Stutzerimonas stutzeri and its application in coking wastewater treatment

KNKhoula NaseerNorth China Electric Power University KAKainat AshfaqUniversity of the Punjab ASAmmara ShamimUniversity of Agriculture Faisalabad

Key Points

To evaluate the aerobic denitrification capability of Stutzerimonas stutzeri KA1 for improving nitrogen removal from coking wastewater.
Isolated Stutzerimonas stutzeri from coking wastewater
Tested nitrate elimination using sodium acetate as a carbon source
Conducted bioaugmentation experiments in a Sequential Batch Reactor
Examined denitrification performance across various dissolved oxygen levels, pH rates, and C/N ratios
Achieved nearly 100% nitrate elimination within 40 hours at optimal pH of 6-10 and C/N ratio of 8
Demonstrated robust denitrification under a wide range of dissolved oxygen levels (0-100%)
Significantly improved nitrogen removal in saline wastewater conditions compared to control systems
Showed effective reduction of Chemical Oxygen Demand (COD), indicating efficient degradation of organic pollutants

Abstract

Improvements in denitrifying bacteria since 1970 have optimized the activity of autotrophic denitrification and heterotrophic nitrification. Synchronous autotrophic biofilms, denitrification, and nitrification have enhanced nitrogen removal from industrial wastewater, but so far, this process relies on variables such as dissolved oxygen, pH, and carbon sources. The aerobic denitrification capability of the coking wastewater-derived Stutzerimonas stutzeri KA1 is quantified in the present investigation to enhance nitrogen removal potential under various environmental conditions. The strain was studied for its ability to isolate nitrate using sodium acetate as the carbon source, and the effectiveness of the microorganism was tested at different dissolved oxygen, pH, and C/N levels. Findings indicate that strain KA1 achieved nearly 100% nitrate elimination within 40 h at pH 6-10 and an optimal C/N ratio of 8, demonstrating resilience under both aerobic and anaerobic conditions. Strain KA1 showed robust denitrification across a wide range of dissolved oxygen levels (0-100%), including stable nitrate reduction under aerobic bulk conditions. Bioaugmentation experiments conducted in a Sequential Batch Reactor (SBR) confirmed that strain KA1 significantly enhanced nitrogen removal, particularly under saline wastewater conditions, exceeding the control systems. In conjunction with nitrogen elimination, the strain also demonstrates robust Chemical Oxygen Demand (COD) reduction, efficiently degrading organic pollutants in coking wastewater. Compared to other studies, Stutzerimonas stutzeri KA1 demonstrated higher denitrification efficiency and greater resistance to environmental stress, making it a scalable, cost-effective solution for denitrifying industrial wastewater. The results offer valuable insights into optimizing wastewater treatment parameters and can advance microbial biotechnology to foster sustainable environmental practices.

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