Throat infections are primarily caused by pathogenic bacteria such as Streptococcus pyogenes , whereas opportunistic fungi such as Candida albicans can infect the oropharyngeal area in conditions of weakened immune systems. Increasing antimicrobial resistance supports the exploration of alternative antimicrobial agents derived from natural sources. Piper crocatum , which is widely used in traditional medicine, contains various bioactive compounds with potential therapeutic properties. This study aims to identify and predict the antimicrobial activity of compounds from P. crocatum through in vitro and in silico studies as natural agents against throat infections. Isolation of compounds was performed using column chromatography by bioactivity‐directed methods and characterized by UV–vis, FTIR, NMR, and MS, and crocatin B (1) was obtained. Antimicrobial activity was tested by disc diffusion and microdilution methods giving strong activity against S. pyogenes (312.5 ± 0.08 μg/mL) and moderate activity against C. albicans (625 ± 0.06 μg/mL). In addition, crocatin B (1) was docked using Autodock 4.0 against the enzyme PBP (−7.19 kcal/mol), SrtA (−5.32 kcal/mol), and CYP51 (−9.60 kcal/mol) to support its activity. Physicochemical and ADMET analyses indicate a profile that crocatin B supports as a lead compound candidate. Therefore, it can be concluded that crocatin B (1) contained in P. crocatum has activity as an antimicrobial agent with the potential to inhibit cell wall pathway and cell membranes of microorganisms that cause throat infections.
Salsabila et al. (Thu,) studied this question.
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