Voltage imaging in zebrafish using high-speed light-sheet microscopy

Voltage imaging in small model animals, such as larval zebrafish, has opened new avenues for understanding how millisecond-scale population neural dynamics drive behaviors. In these animals, multielectrode insertion is technically infeasible, and voltage imaging is the only viable approach for recording spiking activity from many neurons simultaneously. At the same time, the combination of brain transparency and high-speed light-sheet microscopy provides a unique opportunity to apply this technology not only to the brain surface but across the entire brain and spinal cord. Here, we review recent technological advances and neural circuit discoveries made using this technology.