What question did this study set out to answer?

This research aims to elucidate the differences in glioma characteristics related to replication repair deficiency across species and patient subgroups.

April 4, 2026

Abstract 631: Trans-species analysis of central nervous system developmental-specific replication repair deficiency reveals differential patterns of gliomagenesis and response to immunotherapy.

Key Points

This research aims to elucidate the differences in glioma characteristics related to replication repair deficiency across species and patient subgroups.
Developed murine models using germline mutations and Cre-drivers to simulate human RRD subgroups.
Investigated the impact of cell-of-origin on tumor type, location, and onset age.
Characterized the tumor immune microenvironment in forming tumors to assess immune response differences.
Identified significant contributions of the cell-of-origin on brain tumor characteristics (p<0.0001).
Discovered germline mutagenesis onset affects brain tumor development and patient survival (p<0.05).
Characterized subgroup-specific immune landscapes, highlighting CD8+ T cells as key controllers of tumor growth (p<0.0001).

Abstract

Abstract Introduction: Replication repair deficiency (RRD) is a pan-cancer mechanism caused by germline and/or somatically acquired mutations in the replication repair machinery - DNA polymerase proofreading and the mismatch repair (MMR) system. Germline monoallelic (Lynch Syndrome, LS) or biallelic (Constitutional Mismatch Repair Deficiency, CMMRD) mutations in MMR genes are present in 5-10% of glioblastomas in children, adolescents, and young adults. RRD gliomas are lethal, chemoradiation-resistant cancers, characterized by universal hypermutation and variable susceptibility to immune-checkpoint inhibition (ICI). These tumors exhibit variability in patient age of onset, type, location, and response to ICI. Methods: To understand the clinical and biological differences associated with RRD central nervous system (CNS) tumors, we used germline mutations and brain development-specific Cre-drivers to generate murine models that recapitulate the phenotypic and genomic characteristics of each human RRD subgroup: 1) MMRD+PPD (Nestin- and Olig2-Cre+/ Msh2LoxP/LoxP/PoleS459F/+ and LSL-PoleP286R/+): MMR-deficiency (MMRD) in combination with polymerase proofreading deficiency (PPD). 2) MMRD-only (Nestin-Cre+/Trp53LoxP/LoxP and Msh2LoxP/LoxP or Mlh1-/-): MMRD lacking PPD associated with TP53 mutations. Results: Using trans-species comparative approach, we elucidated a mechanistic model of RRD-driven brain tumorigenesis. We revealed that the cell-of-origin significantly contributes to determining brain tumor type, location, and age of tumor onset, suggesting a strong impact of early- or late-RRD mutational onset in shaping tumor biology (p0.0001). Importantly, using murine neural stem cells, we discovered that germline mutagenesis onset directly influences timeline of brain tumor formation and survival between CMMRD and LS patients (p0.05). We further demonstrate the interplay between POLE mutations and MMRD status in modulating the likelihood of brain tumorigenesis in both species. To understand the interaction between hypermutation and the immune system, we characterized the tumor immune microenvironment in spontaneously forming tumors. We uncovered subgroup-specific immune landscapes, with CD8+ T cell activity emerging as a key modulator in controlling brain tumor growth (p0.0001), suggesting an underlying mechanism that may inform therapeutic strategies in RRD patients. Significance: Altogether, our models accurately mimic the human condition, providing a mechanistic framework of RRD-driven brain tumorigenesis, optimization of subgroup-tailored immunotherapy approaches, and putative surveillance protocols. Citation Format: Zoya Aamir, Melissa A. Galati, Emma Gattoni, Owen Crump, Nemanja Ilic, Anirban Das, Nicholas R. Fernandez, Angel K. Wong, Lucie Stengs, Jose R. Dimayacyac, Yuan Chang, Vanessa Bianchi, Melissa Edwards, David Malkin, Cynthia Hawkins, Nuno M. Nunes, Uri Tabori. Trans-species analysis of central nervous system developmental-specific replication repair deficiency reveals differential patterns of gliomagenesis and response to immunotherapy abstract. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 631.

Mark Helpful

Bookmark

Relay