What question did this study set out to answer?

The aim is to develop multiomic long-read sequencing technologies that enhance the understanding of chromatin states and genetic variations.

April 5, 2026

Abstract 3225: Resolving genome regulatory complexity with new multiomic long-read sequencing tools for chromatin state and genetic variation

Key Points

The aim is to develop multiomic long-read sequencing technologies that enhance the understanding of chromatin states and genetic variations.
Development of Fiber-seq for profiling chromatin features directly on DNA
Utilization of DAF-seq to encode chromatin accessibility as mutations through PCR
Comparison of genome coverage between DAF-seq and existing methods like single-cell ATAC-seq
DAF-seq shows nearly a 7,000-fold improvement in genome coverage compared to leading technologies
Fiber-seq provides unbiased multiomic assessment of chromatin accessibility and methylation
Both techniques enable high-resolution profiling from low-input samples, enhancing precision medicine capabilities.

Abstract

Abstract High-resolution epigenomic insights are vital to inform the discovery of biomarkers and mechanisms in cancer research. Modern short-read sequencing (SRS) methods such as ATAC-seq, WGBS, and CUT while DAF-seq enables targeted chromatin accessibility mapping from low-input samples, resolving heterogeneity at single-molecule resolution. These LRS technologies bridge genetic and chromatin regulatory information on single molecules to reveal disease mechanisms, define drug responses, and drive precision medicine. Citation Format: Lu Sun, James T. Anderson, Connor P. Frasier, Allison R. Hickman, Sabrina R. Hunt, Emily A. Madden, Keith E. Maier, Andrea L. Johnstone, Zu-Wen Sun, Martis W. Cowles, Andrew B. Stergachis, Bryan J. Venters, Michael-Christopher Keogh. Resolving genome regulatory complexity with new multiomic long-read sequencing tools for chromatin state and genetic variation abstract. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 3225.

Ask AI

Helpful

Bookmark