Abstract 2254: 3D multimodal spatial profiling of pre-gastric cancer progression using same-slide RNA-protein and fluorescent H&E.

Key Points

• The aim is to identify spatial features that distinguish progressing from non-progressing precancerous niches in gastric tissues.
• Profiled FFPE gastric tissues using a multimodal platform combining RNA and protein analysis.
• Utilized fluorescent H&E staining and 3D holotomography to visualize spatial relationships.
• Applied clustering and classification of transcriptomic and morphological features to define cell states.
• Resolved distinct epithelial, stromal, and immune compartments in precancerous tissue.
• Noted expansion of epithelial and immune states in cancerous regions with altered compartment boundaries.
• Mapped immune cell distributions in relation to gastric epithelium and refined lesion boundaries in 3D.

Abstract