What question did this study set out to answer?

The aim is to develop a glioblastoma spheroid model to study how vascularization affects tumor invasion and responses to therapy.

April 5, 2026

Abstract 7523: A vascularized glioblastoma tumor spheroid model for studying tumor invasion and therapeutic response

Key Points

The aim is to develop a glioblastoma spheroid model to study how vascularization affects tumor invasion and responses to therapy.
Sequential spheroids created by adding U87-MG cells and NHLFs at different stages.
Individual spheroids were formed in hydrogel molds using UV polymerization.
Co-mixed spheroids involved simultaneous addition of U87-MG and NHLFs.
Metabolic activity measured using AlamarBlue on various days.
Comparison of vascularization in sequential vs. co-mixed spheroids.
Sequential spheroids showed peripheral localization of fibroblasts; co-mixed spheroids had uniform distribution.
Sequential spheroids had more vessel formation compared to co-mixed ones.
Higher metabolic activity observed in sequential spheroids on Day 5.
Fibroblast density correlated with sequential spheroid growth.
Temozolomide treatment did not significantly affect sequential spheroid response.

Abstract

Abstract Introduction: GBM is characterized by its extensive invasiveness; its highly infiltrative cells penetrate surrounding brain tissue and often cannot be completely removed through surgery, leading to tumor recurrence and poor survival. The extent of vascularization directly correlates with GBM malignancy. Previous studies have demonstrated that sequentially adding fibroblasts to a pre-formed spheroid resulted in their peripheral concentration and enhanced vascularization in a SN12C kidney cancer model. We are interested in investigating whether sequentially adding fibroblasts promote vasculature formation in vitro and induces angiogenesis in the presence of endothelial cells. The objective of this experiment is to develop a representative model of the GBM tumor spheroid, with a focus on how vascularization and stromal cell populations could affect invasion and therapeutic response. Materials Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 7523.

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