Abstract 3422: Live, label free 3D virtual HE imaging of small intestinal organoids using holotomography and generative cross-modality artificial intelligence

Abstract Organoids provide physiologically relevant 3D models of tumor biology, but current imaging depends on fluorescence labeling, clearing, or destructive sectioning, limiting longitudinal analysis and perturbing native states. Holotomography (HT) enables long-term, high-resolution, label-free 3D imaging of live intestinal organoids, capturing crypt budding, mitosis, apoptosis, and drug-induced cytotoxicity. Generative frameworks that translate refractive index (RI) tomography into multiplexed fluorescence or H virtual H Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 3422.