• AC007637.1 was a novel tumor-suppressive lncRNA in colorectal cancer. • AC007637.1 physically interacts with PCNA and promotes Trim25-dependent PCNA ubiquitination/proteasome degradation. • AC007637.1 regulated DNA damage and repair pathways. Mounting evidence indicates that long noncoding RNAs (lncRNAs) play crucial roles in tumorigenesis and progression. This study aimed to elucidate the role of AC007637.1 , a lncRNA downregulated in colorectal cancer (CRC) identified by our previous transcriptomic analyses. AC007637.1 expression in CRC was assessed via bioinformatic analysis and qRT-PCR. The effects of AC007637.1 on CRC tumorigenesis were evaluated using CCK-8 assays, colony formation assays and tumor xenograft models. RNA immunoprecipitation, RNA pull-down, and co-immunoprecipitation assays were utilized to clarify the molecular mechanism of AC007637.1 in CRC. AC007637.1 serves as a tumor suppressor by inhibiting CRC proliferation and tumorigenicity. It directly binds to proliferating cell nuclear antigen (PCNA) and promotes Tripartite motif-containing 25 (Trim25)-mediated PCNA ubiquitination and subsequent proteasomal degradation, thereby inhibiting DNA replication and repair pathways and enhancing cancer cell sensitivity to DNA-damage-related therapies. In addition, the stability of AC007637.1 is reduced by fragile X-related protein-1 (FXR1) and its transcription is inhibited by promoter hypermethylation. We identify a novel AC007637.1 /Trim25/PCNA regulatory axis in CRC, providing valuable insights into the molecular pathogenesis of this disease. This axis represents a promising therapeutic target for CRC.
Wang et al. (Wed,) studied this question.