What question did this study set out to answer?

The aim is to detail a comprehensive approach for analyzing complex tissue environments through spatial proteomics.

May 3, 2026

Fully Integrated Sample Preparation-Based Spatial Proteomic Analysis.

Key Points

The aim is to detail a comprehensive approach for analyzing complex tissue environments through spatial proteomics.
Protocols for tissue slice preparation and staining are established.
Laser microdissection technology is utilized for collecting specific histological regions.
Integrated spintip-based proteomics and LC-MS techniques are described for sensitive analysis.
Enhanced understanding of tissue microenvironment complex interactions through proteomic analysis.
High sensitivity achieved in identification and quantification of proteins using SISPROT and LC-MS.
Robust methodologies for isolating specific areas of interest from tissue samples.

Abstract

To decipher the complex tissue microenvironment for comprehensively elucidating the biological machinery, spatial proteomics is becoming popular. Laser microdissection (LMD) technology combined with integrated sample preparation and high-sensitive liquid chromatography-mass spectrometry (LC-MS) is the most powerful strategy. In this chapter, we describe the protocols for tissue slice preparation and staining, LMD-based histological regions collection, simple and integrated spintip-based proteomics technology (SISPROT) based sample preparation, and highly sensitive LC-MS analysis.

Mark Helpful

Bookmark

Relay