The functional role of enhancer-promoter configurations in transcription regulation remains poorly understood, despite the wide range of linear genomic distance and relative enhancer positioning observed in endogenous contexts. While canonical models suggest that enhancers operate independently of genomic position, technical limitations have obscured insights on transcriptional kinetics. Here, we employ single-cell MS2/MCP-based live imaging in Drosophila embryos to systematically analyze transcriptional dynamics driven by sub-10kb enhancer-promoter arrangements. Kinetic analyses reveal that while linear enhancer-promoter distance moderately tunes transcriptional output, downstream enhancer positioning reduces mRNA output by 70%. Each configuration modulates distinct transcriptional parameters: linear distance governs initiation kinetics, while relative enhancer positioning dictates transcriptional active state stability. These effects are consistent across varied enhancer and reporter sequences, revealing configuration-dependent cis-regulatory element arrangement as an intrinsic mechanism for transcriptional fine-tuning. This work directly correlates enhancer-promoter configuration with a distinct transcription property for the first time and establishes a framework to dissect the interplay between genome architecture and transcription regulation. Enhancer-promoter configuration influences transcription, but its kinetic basis was unclear. Here, the authors use live imaging in Drosophila embryos to show that distance controls initiation, while downstream enhancers reduce mRNA output by ~70% by destabilising interactions.
Porello et al. (Thu,) studied this question.