Front cover

Key Points

• The central aim is to develop a sensitive sensor for detecting RNase activity using CRISPR-Cas12a technology.
• Utilized CRISPR-Cas12a for amplification of RNase activity detection.
• Incorporated gold nanoparticles to enhance the sensitivity of detection.
• Employed DNA multipliers for signal amplification.
• Demonstrated enhanced sensitivity in detecting RNase activity compared to conventional methods.
• Achieved significant signal amplification through the use of gold nanoparticles.
• Showed potential for practical applications in biosensing.