The SoniConvert system reduced processing time to approximately 30 minutes and achieved cell viability of 80-95% compared to <70% with conventional methods.
The SoniConvert system provides an ultra-fast, high-viability method for isolating adipose-derived stem cells from porcine tissue, significantly improving upon conventional methods.
Adipose-derived stem cells (ADSCs) have emerged as ideal seed cells in regenerative medicine due to their abundant sources, minimally invasive harvesting, multi-lineage differentiation potential, and immunomodulatory properties. Their applications span tissue repair, disease modelling, and cell therapy; however, efficient isolation of high-viability ADSCs remains critical for advancing research and clinical translation. Conventional isolation methods, such as enzymatic digestion combined with mechanical dissociation via pipetting, are limited by lengthy processing times (1-3 h), poor cell viability (often 90% reduction compared to conventional methods); (2) high viability preservation, with trypan blue staining confirming cell viability of 80-95%, exceeding traditional protocols; and (3) broad compatibility, as the resulting single-cell suspensions meet requirements for primary cell culture, flow cytometry, cytotoxicity assays, and adipose organoid construction. By reducing processing time, enhancing cell integrity, and limiting variability, this system provides a practical platform for adipose-related research.
Xiao et al. (Tue,) reported a other. SoniConvert system vs. Conventional isolation methods was evaluated on Processing time and cell viability. The SoniConvert system reduced processing time to approximately 30 minutes and achieved cell viability of 80-95% compared to <70% with conventional methods.
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