AVITHRAPID - Standard Operating Procedure - Antiviral activity evaluation by CPE – Virucidal compounds

Key Points

• This study aims to assess the antiviral activity of compounds using a method based on cytopathic effects in a specific cell line.
• Evaluated antiviral effects on SARS-CoV-2, WNV, and CHIKV infections in the Vero E6 cell line.
• Determined cellular toxicity by testing doses allowing greater than 90% cell viability.
• Utilized a low-cost CPE method for initial antiviral efficacy assessments.
• Cytopathic effects observed in Vero E6 infected by SARS-CoV-2, WNV, and CHIKV, validating the method.
• The highest dose tested showed >90% cell viability, ensuring reliable antiviral assessment.