What does this research mean for the field?

The filamentous fungus Arcopilus aureus possesses a 33.77 Mb nuclear genome organized into seven chromosomes with 10,282 protein-coding genes, and a 33.8 kb circular mitogenome. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This research aims to generate a comprehensive chromosome-level assembly of the nuclear and mitochondrial genomes of Arcopilus aureus.

June 5, 2026Open Access

Chromosome-level de novo assembly of the nuclear and mitochondrial genomes of Arcopilus aureus, a filamentous fungus with multifaceted ecological and economic roles

Key Points

This research aims to generate a comprehensive chromosome-level assembly of the nuclear and mitochondrial genomes of Arcopilus aureus.
Integrated next-generation sequencing, PacBio HiFi, and Hi-C technologies for genome assembly.
Characterized the nuclear genome spanning 33.77 Mb and mitogenome of 33,820 bp.
Identified 10,282 protein-coding genes and major RNA components in the genomes.
The nuclear genome was organized into seven chromosomal-sized scaffolds with an N50 of 5.09 Mb and BUSCO completeness of 95.91%.
The mitogenome contains 15 protein-coding genes and multiple tRNAs and rRNAs, contributing to its cellular functions.
The assembly provides insights into the ecology and evolutionary mechanisms of A. aureus, including its roles in biocontrol and metabolite synthesis.

Abstract

The filamentous fungus Arcopilus aureus (Sordariale: Chaetomiaceae) is notable for its multi-domain significance across agriculture, medicine, and industry. In this study, we generated a chromosome-level nuclear genome and a complete circular mitogenome for A. aureus by integrating data from next-generation sequencing, PacBio HiFi, and Hi-C technologies. The final nuclear genome assembly spans 33.77 Mb (GC content: 57.67%), and was organized into seven chromosomal-sized scaffolds (only one gap) with an N50 size of 5.09 Mb and BUSCO completeness of 95.91%. A total of 10,282 protein-coding genes, 228 non-coding RNAs, and ~1.77 Mb of repetitive elements were predicted in the nuclear genome. By contrast, the mitogenome of A. aureus is 33,820 bp in length, with a GC content of 25.96%. It harbors 15 typical mitochondrial protein-coding genes, one unidentified ORF, two rRNAs (small subunit rns and large subunit rnl), and 28 tRNAs. This high-quality genome assembly provides a valuable resource for understanding the ecology, genetics, and evolution of A. aureus, which facilitates elucidating its mechanisms of biocontrol, infection, and metabolite synthesis.

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