A protocol for isolating single nuclei from fresh-frozen murine cardiac ventricular tissue enables integrated multiomic analysis of gene expression and chromatin accessibility.
This protocol provides a standardized method for isolating single nuclei from murine cardiac tissue, facilitating advanced multiomic and epigenomic research in cardiology.
Pathological cardiac remodeling involves cell-type-specific regulatory changes that require integrative analysis across all cardiac cell populations. Here, we present a protocol for isolating single nuclei from fresh-frozen murine cardiac ventricular tissue to enable the integrated analysis of gene expression and chromatin accessibility. We describe steps for mechanical homogenization, sequential filtration, sucrose cushion purification, and fluorescence-activated nuclei sorting (FANS). This protocol enables multiomic analysis across various cardiac cell types and supports epigenomic studies of gene regulation.
Veras et al. (Mon,) conducted a other in Pathological cardiac remodeling. Protocol for isolating single nuclei from fresh-frozen murine cardiac ventricular tissue was evaluated. A protocol for isolating single nuclei from fresh-frozen murine cardiac ventricular tissue enables integrated multiomic analysis of gene expression and chromatin accessibility.
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