What does this research mean for the field?

A combined day-and-night facial serum regimen containing L-ascorbic acid, proteoglycans, hyaluronic acid, Vigna aconitifolia extract, and melatonin is well-tolerated and significantly improves skin texture and firmness. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

The study aims to assess the impact of a dual facial serum regimen on skin aging and its safety in adult participants.

June 7, 2026Open Access

An Open-Label Pilot Study Exploring Skin Quality Changes and Safety of a Day-and-Night Facial Serum Combination Containing L-Ascorbic Acid, Proteoglycans, Hyaluronic Acid, Vigna aconitifolia Extract, and Melatonin

Key Points

The study aims to assess the impact of a dual facial serum regimen on skin aging and its safety in adult participants.
Single-arm, non-randomized, open-label study with 30 participants aged 35-55 years.
Participants applied day and night facial serums for 8 weeks, with assessments at multiple time points.
Objective measures included skin texture, elasticity, firmness, hydration, melanin index, and TEWL.
Significant improvements in skin texture (p = 0.002) and reduction in skin depression (−22.7%, p < 0.001) after 2 months.
Skin firmness increased significantly at 1 month (p < 0.001) and remained elevated post-treatment (p = 0.008).
Mild transient burning was the common adverse effect, with other non-serious events reported.

Abstract

Background: A novel day–night facial serum regimen combining antioxidants, hydration-enhancing agents, and bioactive compounds has been developed to address skin aging. Objective: The aim is to explore changes in skin aging parameters and assess the safety and tolerability of a day-and-night facial serum combination. Methods: In this single-arm, non-randomized, prospective, open-label study, 30 participants aged 35–55 years applied a day facial serum (DFS) and a night facial serum (NFS) for 8 weeks. Objective assessments included skin texture and depression (Antera 3D®), elasticity and firmness (Cutometer®), hydration (Corneometer®), TEWL (Tewameter®), melanin index (Mexameter®), and brightness (Colorimeter®). Evaluations were performed at baseline, at 1 month and 2 months after treatment start, and at 1 month post-treatment. Results: Thirty participants (Fitzpatrick III–IV) completed the study. Significant improvements in skin texture (p = 0.002) and reduction in skin depression (−22.7%, p < 0.001) were observed after 2 months. Skin firmness increased significantly at 1 month (p < 0.001) and remained elevated post-treatment (p = 0.008). The melanin index decreased at 1 month (p = 0.048), while hydration declined after discontinuation (p = 0.016). TEWL showed a significant overall time effect; however, no Bonferroni-adjusted pairwise comparison versus baseline was significant. No significant changes were observed in elasticity or brightness. Mild transient burning was the most common adverse event. Other reported adverse events included acne, miliaria rubra, oiliness, and mild itching; all were non-serious and did not result in treatment discontinuation. Conclusions: The combined DFS and NFS regimen was well tolerated and was associated with favorable changes in skin texture and firmness. Transient local reactions, particularly mild burning sensation, were commonly reported but did not result in treatment discontinuation. Further controlled studies are warranted to confirm these observations.

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