UV-Visible spectroscopy is a widely used analytical technique in various fields such as pharmaceuticals, environmental science, food industry, and materials science. It explores the methodologies employed in the development and validation of UV-Visible spectroscopic methods. The focus is on discussing the key steps involved in method development, including selection of appropriate solvent, determination of suitable wavelength, optimization of instrumental parameters, and validation of the developed method according to regulatory guidelines. A significant portion of the review is dedicated to method validation, focusing on parameters such as accuracy, precision, linearity, robustness, etc. In addition to method development and validation, the review explores various estimation techniques utilizing UV-Visible spectroscopy. To develop and validate a UV-spectrophotometric approach for estimating the formulation of the anti-hyperlipidaemic drug- Fenofibrate. An effective, accurate, precise, and reproducible UV-spectrophotometric method in ethanol was developed for the validation of drug. ethanol was used as the medium for the UV-spectrophotometric measurement, which was carried out with a UV-Vis spectrophotometer. LOD and LOQ, selectivity and specificity, linearity and range, and accuracy, and precision were performed as validation parameters.
D. Sravanthi*1, M. Divya Sai, J. Pujitha, A. Laxmi Kavitha (Sun,) studied this question.