The present publication continues the already derived geometric-temporal line from the structural water window toward biological compatibility. Previous works showed that water should not be treated as a passive solvent, but as an ensemble of admissible structural-dynamic regimes whose temperature redistribution gives rise to an intermediate water window, a broad life-compatible regime, and a narrower enzyme-compatible regime 1-3. The present work does not repeat that derivation, but examines the next functional narrowing: the emergence of a DNA-enzyme biological temperature window. The main question is whether the already derived water regime - organized but reconfigurable - can be understood as a condition for meeting between DNA grooves and enzymatic/protein regimes. In this reading, DNA is not introduced as a dry molecular structure, but as a hydrated biological interface whose readability depends on groove geometry, boundary conditions, the ion-hydration regime, and local water architecture. Enzymes and DNA-binding proteins are not treated only as autonomous molecular objects, but as regimes that can bind, orient, and support transition only if interfacial water is in a suitable structural-dynamic temperature regime. It is shown that temperature does not act only as a kinetic accelerator. It maintains or disrupts the state of water, on which both the hydration readability of DNA grooves and the enzymatic transition-supporting compatibility depend. At too low temperature, water may become overly domain-organized and weakly reconfigurable. At too high temperature, it may become too noisy and short-lived to support precise interfacial selection. The intermediate biological window arises as the region in which water remains sufficiently stable to hold a hydrated interface and sufficiently dynamic to allow binding, orientation, and transition. Thus the biological temperature window is formulated not as the maximum of a single factor, but as an overlap of admissibilities between the water state, the hydration readability of DNA grooves, and the enzymatic ability for specific binding and catalytic transition. External sources are used only as a comparative layer with respect to the already derived logic, not as its starting basis.
Balevsky et al. (Wed,) studied this question.