AIMS: and evaluate the contribution of human, animal, food, and environmental strains to the dissemination of antimicrobial resistance and virulence traits within a One Health framework. MATERIALS AND METHODS: A total of 975 publicly available genomes were analyzed using comparative genomics to characterize the resistome, virulome, and mobilome. Associations between antimicrobial resistance genes and mobile genetic elements were assessed. Ribosomal multilocus sequence typing (rMLST) was used to investigate population structure and lineage distribution across sources and geographic regions. RESULTS: showed a global distribution across diverse hosts. A subset of rMLSTs (48500, 48501, 48492, and 48498) accounted for ~52% genomes and were widely distributed across countries and sources. Multidrug resistance was detected in all regions and frequently associated with plasmids, prophages, and integrative and conjugative elements, which together carried nearly half of resistance genes. In contrast, virulence determinants were largely chromosomal and conserved, supporting a stable pathogenic repertoire across ecological contexts. CONCLUSIONS: These findings highlight the circulation of dominant lineages across multiple reservoirs and identify non-clinical environments as important contributors to the spread of clinically relevant resistance and virulence traits.
Silva et al. (Tue,) studied this question.