What question did this study set out to answer?

The study aims to quantify plasma proteins to understand differences in heritable thoracic aortic disease, particularly in Loeys-Dietz Syndrome.

July 9, 2026

Dysregulated Proteins in Plasma Distinguishing Loeys-Dietz Syndrome from Other Heritable Thoracic Aortic Disease – An Explorative Study

Key Points

The study aims to quantify plasma proteins to understand differences in heritable thoracic aortic disease, particularly in Loeys-Dietz Syndrome.
Analyzed plasma samples from 7 patients with ACTA2 pathogenic variants and 16 healthy controls.
Used targeted proteome analysis of 276 proteins via immunoaffinity proteomics.
Adjusted for confounders to compare protein levels between groups.
Oncostatin M and Pentraxin 3 levels were generally higher in HTAD patients.
Specifically, higher levels of TNFRSF9, GNLY, GP1BA, and CD5 were found in LDS patients compared to controls.
These proteins correlated positively with Th17 and platelet counts.

Abstract

OBJECTIVES: Thoracic aortic aneurysms (TAA) are often found in younger individuals and approximately 20% may be associated with heritable thoracic aortic disease (HTAD). There are some data on genomic biomarkers reflecting inflammation and extracellular matrix remodeling in HTAD. However, data that accurately reflect the corresponding protein changes are scarce. Our aim was to quantify proteins by using targeted proteomics in HTAD patients versus healthy controls, to better understand the underlying pathophysiology. METHODS: actin alpha 2 (ACTA2, n = 7) pathogenic variants were recruited at our outpatient clinic. For comparison, blood samples were drawn from 16 healthy controls. Plasma samples were analyzed by targeted proteome analysis of 276 proteins using immunoaffinity proteomics. RESULTS: Whereas Oncostatin M and Pentraxin 3 levels appeared generally higher in HTAD patients, after adjusting for several confounders, significantly higher levels for these markers as well as TNF Receptor Superfamily Member 9 (TNFRSF9), Granulysin (GNLY), CD5, Vasorin and Glycoprotein 1b-α (GP1BA) were only observed in LDS patients compared to healthy controls. Levels of TNFRSF9, GNLY, GP1BA and CD5 correlated positively with Th17 and platelet counts. CONCLUSIONS: This discovery study suggest that LDS could represent a particular inflammatory subgroup of HTAD patients potentially reflecting the involvement of Th17 and platelet related mechanisms in the progression of TAA. Larger studies are needed to evaluate if the identified proteins could be used as biomarkers in these patients.

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