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The study aims to isolate and characterize new anti-inflammatory compounds from Viburnum macrocephalum leaves.

July 27, 2026Natural Product Research0 citations

Isolation, characterisation, and anti-inflammatory activities of constituents from Viburnum macrocephalum f. macrocephalum leaves

Key Points

The study aims to isolate and characterize new anti-inflammatory compounds from Viburnum macrocephalum leaves.
Isolated three new iridoids from Viburnum macrocephalum leaves through phytochemical investigation.
Determined the structural characteristics using 1D/2D NMR and HRESIMS.
Assessed the anti-inflammatory effects by measuring nitric oxide suppression in RAW264.7 macrophages.
Compound 2 significantly suppressed nitric oxide generation (IC50 = 5.39 μM).
Molecular docking indicated compound 2 binds effectively to iNOS/COX-2 active sites.
VMML demonstrated promising anti-inflammatory activity through its constituents.

Abstract

From Viburnum macrocephalum f. macrocephalum leaves (VMML), three new iridoids, viburetins A–C (1–3), together with four previously reported constituents, were isolated following a phytochemical investigation. Structural elucidation of the new isolates was achieved via detailed spectroscopic analyses (1D/2D NMR, HRESIMS) coupled with ECD experiments. The suppression of nitric oxide (NO) generation in RAW264.7 murine macrophages stimulated by LPS was assessed to determine anti-inflammatory effects of these isolates. Notably, compound 2 exhibited potent suppressive effects on NO generation (IC50 = 5.39 μM). Furthermore, molecular docking studies showed that compound 2 could effectively bind to the catalytic cavities of iNOS/COX-2 via hydrogen bonds and hydrophobic interactions. These results suggested that VMML containing these constituents possessed promising anti-inflammatory activity.

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