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Abstract A general method is described for the quantitative determination of the binding of glycoproteins, and their enzymatically modified derivatives, to the plasma membranes of rat liver. The assay is based upon the competitive inhibition of the binding phenomenon in an operationally irreversible system. Utilizing this procedure, a quantitative estimate of the ability of the membranes to bind four normally circulating serum glycoproteins, as well as their asialo and agalacto derivatives, has been made. The results obtained extend the previous qualitative observations in vivo and provide the basis for a systematic analysis of the structural determinants involved in the binding process.
Lenten et al. (Sat,) studied this question.
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