Purified EV characterization and confirmation of sEV uptake in tissue. A and B, transmission electron micrograph of sEVs from OVCAR3 (A) and FT240 (B) cells under 30,000× magnification. C, Western blots comparing the expression of sEV markers CD9, CD81, and FLOT1 in FT240 and OVCAR3 cell lysates and purified sEVs. Purified sEVs show enrichment of EV markers. D, Size distribution of OVCAR3 and FT240 sEVs determined using NTA. E, Venn diagram showing the number of proteins identified in FT240 and OVCAR3 sEVs as well as the overlap between samples. F, Confocal fluorescent imaging of mCherry-labeled FT240 cell–derived sEV uptake (2 μg) in fallopian tube cells. G, Bar plot quantifying uptake of mCherry in fallopian tube cell line (F). Each point represents an individual cell. H, IHC staining for mCherry after human fallopian tube tissue incubation with 1.51E10 mCherry-labeled FT240 cell–derived sEV for 24 hours.
Sipes et al. (Mon,) studied this question.