Pancreatic neuroendocrine tumors (PanNETs) exhibit heterogeneous clinical behavior, and a growing number of NF-PanNETs have been discovered incidentally. While chromatin remodeling and telomere maintenance gene alterations, such as ATRX and DAXX mutations, are well-established in the metastatic progression of PanNETs, many tumors lack known driver mutations. To identify additional prognostic biomarkers and alternative oncogenic mechanisms in primary non-functional pancreatic neuroendocrine tumors (NF-PanNETs), we employed whole transcriptome sequencing (WTS) on 73 non-syndromic NF-PanNETs with extended clinical follow-up (>4 years). Findings were validated via immunohistochemistry in an independent multi-institutional cohort of 539 PanNETs. Clinicopathologic correlation and survival analyses assessed the prognostic significance of identified biomarkers. Transcriptomic profiling identified six distinct clusters, with Cluster 6 (C6) demonstrating aggressive features, including high WHO grade and distant metastases. Gene ontology pathway analysis of C6 tumors revealed upregulation of protein homeostasis, immune regulation, insulin metabolic activity, and telomere maintenance via telomerase activation. Recurrent fusion genes involving the chromatin remodeling gene, BEND2 (CHD7::BEND2 and EWSR1::BEND2) were detected in 7% (5/73) of NF-PanNETs, exclusively in C6 and independent of ATRX/DAXX status. Orthogonal validation showed BEND2 expression in 3% (16/539) of PanNETs, all harboring BEND2 fusion genes by WTS. Patients with BEND2-positive tumors had significantly shorter disease-free survival (DFS, p<0.001) and disease-specific survival (DSS, p<0.001). Further, multivariate analysis confirmed BEND2 as an independent negative prognostic factor for DFS (p<0.001) and DSS (p=0.001). Overall, ATRX, DAXX, and BEND2 alterations were present in 62% of metastatic NF-PanNETs. This study identifies recurrent BEND2 fusion genes as a novel oncogenic mechanism in aggressive NF-PanNETs and establishes BEND2 expression as an independent prognostic biomarker, emphasizing the importance of chromatin remodeling and telomere maintenance in the metastatic progression of NF-PanNETs.
Wood et al. (Fri,) studied this question.