Abstract Microfluidic organ‐on‐chip (OoC) devices replicate physiological conditions in vitro, making them valuable for disease modeling and drug screening. Traditional OoC fabrication relies on microfabrication and polydimethylsiloxane (PDMS) replica molding, which are costly and time‐consuming. 3D printing offers an attractive solution to directly produce biocompatible, assembly‐free microfluidic OoC devices. Although researchers have demonstrated the use of light‐based 3D printing techniques to produce unibody microfluidic OoC devices, broader adoption within the OoC community requires lowering the technical and logistical barriers, especially for those new to 3D printing. Commercial desktop printers and off‐the‐shelf resins are instrumental in facilitating the transition from PDMS replica molding to 3D printing, as they streamline material preparation and printing workflows. However, their application in the context of microfluidic OoC development remained underexplored. This work aims to bridge this gap by providing a practical framework to fabricate microfluidic devices that support different cell culture configurations using a selected commercial resin and its manufacturer's recommended printing parameters. In addition to 2D monolayer culture, can directly print functional structures, such as micropillars and porous membranes, to support 3D spheroid and air‐liquid interface barrier cultures, respectively. By leveraging these off‐the‐shelf solutions, the entry barriers are lowered to harnessing 3D printing technologies for OoC research.
Ong et al. (Tue,) studied this question.