Post-partum supplementation of calcium salts of long-chain fatty acids and fibrolytic enzymes on reproductive performance and blood metabolites of lactating Surti buffaloes

Twenty-four lactating Surti buffaloes (BW=459.21±7.39 kg and parity of 2.58±0.15) after seven days of calving were randomly divided into four treatment groups. The control (CON) group was fed with basal diet (chaffed sorghum straw, green fodder hybrid napier and BIS Type-I compound cattle feed). The second group was fed the exogenous fibrolytic enzymes (EFE) supplement, while the third and fourth groups (Bypass Fat-1 and Bypass Fat-2)were fed EFE along with 1% and 2% of bypass fat of total DMI, respectively. Blood biochemical parameters were measured at the start (0 d), middle (75th d), and end (150th d) of the experiment. The treatments had no significant effect on the serum levels of glucose, total protein, T3, T4, urea, and lipid profile (including triglycerides, total cholesterol, HDL, LDL, VLDL and NEFA). However, animals that received these supplements reduced service period and fewer services per conception when compared to the control group. Thus, adding bypass fat and fibrolytic enzyme supplements to animals’ ration could improve their reproductive performance without negatively impacting their blood biochemical indicators.