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This is a Experimental Study study.

September 28, 2025

Sanger Tree of Life HMW DNA Extraction: Manual Tissue Nanobind® v2

Key Points

Manual extraction of high molecular weight DNA yields high quality and quantity for sequencing needs.
This protocol ensures effectiveness across various animal species, including mammals and birds, for DNA studies.
Utilization of the Nanobind PanDNA kit enhances the extraction process from animal tissue specimens.
The output can be processed further with the Diagenode Megaruptor 3 or Opentrons OT-2 for fragmentation.

Abstract

This protocol describes the manual extraction of HMW DNA from animal tissue samples intended for long-read sequencing using the Nanobind® PanDNA kit and and following the ‘Extracting DNA from animal tissue using Nanobind® PanDNA kit’ procedure from PacBio. This process is effective for several animal species covered by the Tree of Life Programme, including molluscs, mammals, birds, fish and amphibians. The output of this protocol is high quality and quantity HMW DNA, which can be directed towards the Sanger Tree of Life HMW DNA Fragmentation: Diagenode Megaruptor® 3 for LI PacBio or the Sanger Tree of Life HMW DNA Fragmentation: Opentrons® OT-2 for PacBio LI protocols. Acronyms HMW: high molecular weight LI: low input

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Sanger Tree of Life HMW DNA Extraction: Manual Tissue Nanobind® v2

Key Points

Abstract

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