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Massive infiltration of T cells and tumor cell lysis in the tumor tissues by the treatment with 7 × 19 CAR‐T. NOG‐ΔMHC mice were inoculated subcutaneously with 3.5 × 106 U87MG EGFRvIII cells on day 0, followed by intravenous injection of 1 × 107 UTD, Conv. CAR‐T, or 7 × 19 CAR‐T cells on day 10. Tumor tissues were resected from the mice on day 13 to prepare formalin-fixed, paraffin-embedded slices for H&E and IHC staining using rabbit anti–human CD4, CD8, CCR7, caspase-3 mAbs and mouse anti–human EGFRvIII mAb. Stained cells were visualized and observed by microscopic examinations at ×100 and ×400 magnifications. A, Representative images are displayed. Scale bar indicates a length of 200 μm (×100) or 50 μm (×400). B, The number of stained cells per tumor area (mm2) or the percentage of the stained area was calculated using BZ‐X analyzer software. Data are shown as mean ± SD of triplicate samples (n = 3 each per group, biological replicates). *, P P P
Ohta et al. (Wed,) studied this question.