RNA Isolation with TRIzol™ (Invitrogen) and on column DNase treatment using Qiagen RNeasy v1

This protocol is a compilation of the protocols for isolating total RNA from frozen tissue using TRIzol (Invitrogen) and DNase treating total RNA using the Qiagen RNA clean up protocol. The text and workflow have been largely taken and adapted from the manufacturers standard protocols from Invitrogen and Qiagen. The resulting RNA is suitable for most downstream applications including RNA-seq and qRT-PCR. Original manufacturer protocols can be found at: TRIzol (Invitrogen): https: //assets. thermofisher. com/TFS-Assets/LSG/manuals/trizolᵣeagent. pdf Qiagen RNeasy: https: //www. qiagen. com/us/products/discovery-and-translational-research/dna-rna-purification/rna-purification/total-rna/rneasy-kits