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Abstract The mechanisms that regulate Ewing sarcoma (EWS) cell dissemination are poorly characterized. We have reported that though cell line studies show the EWSR1::FLI1/ERG fusion oncoproteins represses ETS1 expression, there is inter-tumoral variations in its mRNA levels and higher expression of ETS1 in tumors correlate with reduced overall patient survival. ETS1 encodes a transcription factor that regulates the movement of several early progenitor cell sub-types, and activation of ETS1 in EWS cells enhanced their movement and migration. This suggests that ETS1 expression could promote cell dissemination. We also showed that ETS1 expression in EWS cells upregulated the expression of the focal adhesion (FA) protein TENSIN3 (TNS3). Critically, depletion of TNS3 reverted the enhanced formation of FAs induced by ETS1 and partially rescued the increased migratory phenotype. In this study, we investigated the ETS1-induced changes in the phenotype of EWS cells by assessing TNS3 function in the context of other FA proteins. We generated epigenetic and transcriptomic profiles of EWS cells using ChIP-seq, CUT 2024 Sep 5-8; Toronto, Ontario, Canada. Philadelphia (PA): AACR; Cancer Res 2024;84(17 Suppl):Abstract nr A004.
Ebegboni et al. (Thu,) studied this question.