Single-Crystal Structural Analysis of 2D Metal–Organic Frameworks and Covalent Organic Frameworks by Three-Dimensional Electron Diffraction

ConspectusIn the development of 2D metal–organic frameworks (MOFs) and 2D covalent organic frameworks (COFs), obtaining structural details at the atomic level is crucial to understanding their properties and related mechanisms in potential applications. However, since 2D-MOFs and COFs are composed of layered structures and often exhibit sheet-like morphologies, it is challenging to grow large crystals suitable for single-crystal X-ray diffraction (SCXRD). Therefore, ab initio structure determination, which refers to solving the structure directly from experimental data without using any prior knowledge or computational input, is extremely rare for 2D-MOFs and COFs. In contrast to SCXRD, three-dimensional electron diffraction (3DED) only requires crystals sized in tens or hundreds of nanometers, making it an ideal method for single-crystal analysis of 2D-MOFs and COFs and obtaining their fine structural details.In this Account, we describe our recent development of the 3DED method and its application in structure determination and property studies of 2D-MOFs and COFs. A key development is the establishment of a continuous 3DED data collection protocol. By collecting electron diffraction (ED) patterns continuously while performing crystal tilting, the electron dose applied to the target nanocrystal is greatly reduced. This allows the acquisition of high-resolution 3DED data from 2D-MOFs and COFs by minimizing their damage under the electron beam. We have also developed an approach to couple 3DED with real-space structure solution methods, i.e., simulated annealing (SA), for single-crystal structural analysis of materials that do not have high crystallinity. We successfully determined two 2D-COF structures by combining 3DED with SA.We provide several examples demonstrating the application of 3DED for the ab initio structure determination of 2D-MOFs and COFs, revealing not only their in-plane structures but also their stacking modes at the atomic level. Notably, the obtained structural details serve as the foundation for further understanding the properties of 2D-MOFs and COFs, such as their electronic band structures, charge mobilities, etc. Beyond structure determination, we describe our work on using 3DED as a high-throughput method for the discovery of new materials. Using this approach, we discovered a novel MOF that was present only in trace amounts within a multiphasic mixture. Through this discovery, we were able to tune the synthesis conditions to obtain its pure phase.We detail how 3DED can be used to probe different levels of molecular motions in MOFs through the analysis of anisotropic displacement parameters (ADPs). Additionally, we show that 3DED can provide accurate information about intermolecular weak interactions such as hydrogen bonding and van der Waals (vdW) interactions. Our studies demonstrate that 3DED is a valuable method for the structural analysis of 2D-MOFs and COFs. We envision that 3DED can accelerate research in these fields by providing unambiguous structural models at the atomic level.