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Digital holography is an imaging technique that enables a 3-dimensional reconstruction of both the amplitude and phase of an electromagnetic field after its interaction with an object. Second harmonic generation being a coherent process, it can also be used to generate interferences and holograms. Since collagen molecules exhibit a significant second order response, we apply harmonic holography to porcine cornea samples to obtain single-shot measurements of the 3D spatial distribution of the collagen fibers. In addition, we propose polarization multiplexing to study the polarization dependence of the sample response.
Goldmann et al. (Tue,) studied this question.
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