Development and Validation of UV Spectrophotometric Method for the Determination of Ibuprofen by Using Quality by Design (QbD) Approach

Objective: To develop a simple, rapid, accurate, robust and inexpensive spectrophotometric method for the estimation of Ibuprofen by using quality by design (QbD)" approach.Methods: A UV spectrophotometric method was developed on the Systronics AU-2701 UV-visible double beam spectrophotometer using ethanol and water as solvents.A wavelength of 228 nm was selected as absorbance maxima (max).The effect of input variables on spectrum characteristics was studied for the selection of critical parameters and the proposed method was validated for various parameters like system suitability, linearity, precision, accuracy, detection limits and quantification limits as per the International Conference on Harmonization guidelines ICH Q2(R1). Results:The linearity of the method was found to be excellent over the concentration range of 5 to 25 µg/ml with a high correlation coefficient value of 0.9998.The detection and quantification limits were 0.59 µg/ml and 1.80 µg/ml respectively.The mean recovery was found to be 97.83% with a low percentage relative standard deviation (% RSD) value.The precision study also has shown a low % RSD value (<1).No interfering peaks were observed during specificity studies. Conclusion:The obtained result indicated that the developed spectrophotometric method is simple, rapid and accurate for determining Ibuprofen.