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You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Prostate & Genitalia (MP11)1 May 2024MP11-17 WATER-AVOIDANCE STRESS AGGRAVATES PROSTATIC INFLAMMATION IN A NOVEL MURINE MODEL OF CHRONIC PROSTATITIS Jianshu Ni Jianshu NiJianshu Ni View All Author Informationhttps://doi.org/10.1097/01.JU.0001008564.85995.11.17AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Psychological stress has been recognized to aggravate symptoms in patients with chronic prostatitis (PRO). To date, few studies have considered the influence of psychological factors on PRO models. Here, we aimed to establish a novel model combining chemically induced prostatitis with psychological stress. METHODS: A total of 40 mice were randomly divided into four groups: normal control (NC) group, PRO group, water avoidance stress (WAS) group and PRO + WAS group. Ten mice were assigned to each group: five for cystometrography (CMG) and five for von Frey testing and histological analysis. Chronic prostatitis was induced through a prostatic injection of 10% paraformaldehyde (PFA). The WAS mice were placed on the middle platform for 1 hour per day for ten consecutive days. RESULTS: These CMG results suggested that the PRO group, the WAS group and the PRO + WAS group all exhibited bladder overactivity symptoms and hyperexcitability of the micturition reflex, which presented as a shortened micturition interval and decreased threshold pressure evoking bladder contraction. The symptoms of the PRO group and the PRO + WAS group were more severe than those of the WAS group. The tissue staining results indicated that WAS itself caused only mild prostatic inflammation but could significantly aggravate chemical-induced prostatic inflammation, as well as the total number of mast cells and proportion of activated mast cells. The results of the von Frey test demonstrated that both WAS and PRO induced bladder hyperalgesia in mice, and WAS slightly aggravated the pelvic pain symptoms induced by PRO. CONCLUSIONS: We established a novel murine chronic prostatitis model by combining chemical-induced prostatitis with psychological stress, and the model mice manifested persistent bladder overactivity, pelvic hyperalgesia and prostatic inflammation. Histological analysis further indicated that WAS itself induced mild prostatic inflammation, but could significantly aggravated primary prostatic inflammation. Download PPTDownload PPT Source of Funding: This study is supported by Interdisciplinary Program of Shanghai Jiao Tong University (project number YG2021QN74), the National Natural Science Foundation of China (No. 82100818) and the Shanghai Sailing Program (No. 21YF1423400) © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e157 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Jianshu Ni More articles by this author Expand All Advertisement PDF downloadLoading ...
Jianshu Ni (Mon,) studied this question.