Existing Drosophila exercise models only distinguish between regular and endurance exercise based on behavioral traits and have not yet established an exercise physiology classification system based on metabolic traits, which restricts the depth of application of this model organism in exercise physiology research. This study aims to establish an aerobic/anaerobic metabolic model of Drosophila by constructing an incremental load exercise model. Method: A 7-day-old wild-type Drosophila was used as the study subject for the exercise intervention using a Drosophila exercise device. Six exercise intensity gradients were used in this study: 0.31 rev/s (E1), 0.45 rev/s (E2), 0.59 rev/s (E3), 0.71 rev/s (E4), 0.83 rev/s (E5), and 0.91 rev/s (E6). Drosophila in all exercise groups were subjected to a single acute exercise session of 2.5 h. No exercise intervention was performed in the control group. At the end of the exercise, the expression levels of anaerobic metabolizing enzymes (Hex-A, Hex-C, Pfk, Pyk, Ldh) and aerobic metabolizing enzymes (dPdh-A, dPdh-B, Idh, Nc73EF, whd) in Drosophila were detected by RT-qPCR. Meanwhile, the type of aerobic and anaerobic metabolism during Drosophila exercise was comprehensively analyzed by measuring Drosophila blood lactate concentration, respiratory quotient, mitochondrial function, and alginate concentration. 1) Anaerobic metabolism key enzyme activity showed a peak response at E5 loading intensity, and aerobic metabolism marker enzyme activity reached the activation threshold at E2 intensity; 2) blood lactate kinetic curve showed that the overall concentration was significantly higher than that of the control group (P < 0.05), and a significant inflection point at E5 intensity; 3) a significant increase in the respiratory quotient occurred when the loading intensity was elevated to E5 and E6 (P < 0.01); 4) Trehalose concentration decreased dose-dependently with loading intensity; 5) Mitochondrial maximal respiratory capacity was enhanced with exercise loading, and a significant elevation of E5 intensity occurred (P < 0.05), and E6 intensity entered a plateau; 6) The metabolic effects produced by E5 and E2 loading interventions were homologous to the characteristics of human aerobic/anaerobic exercise benefit. Drosophila showed a dominant anaerobic metabolic phenotype at 0.83 rev/s (E5) loading intensity, while 0.45 rev/s (E2) intensity showed a typical aerobic metabolic pattern.
Yi et al. (Thu,) studied this question.