Organ-specific profiles of very-long-chain aliphatic wax constituents in Hyacinthella leucophaea

Cuticular waxes form the outermost protective barrier of plant organs, and these waxes are composed primarily of very-long-chain aliphatic compounds that mitigate water loss and shield tissues from biotic and abiotic stress. Despite their ecological and functional significance, the surface wax chemistry of Hyacinthella leucophaea has not previously been characterized. In this study, flowers and leaves of H. leucophaea were analyzed. GC-MS analysis of flower wax washings revealed dominance of long-chain n-alkanes and monoketones, with palmitone accounting for over 40% of the total wax and n-hentriacontane representing the major hydrocarbon. Leaf waxes are characterized by high proportions of very-long-chain primary alcohols, substantial amounts of n-alkanes, and minor levels of aldehydes and esters. Both organs shared hentriacontane as the principal n-alkane yet displayed markedly different accompanying compound classes. The contrasted profiles, with ketone-rich floral waxes and alcohol-rich leaf waxes, highlight significant metabolic divergence between tissues. This work provides the first detailed characterization of H. leucophaea cuticular waxes and contributes to a broader understanding of structural lipid diversity in geophytic taxa.