Bordetella saccharivorans sp. nov., Isolated from Leaf Soil

An aerobic, oligotrophic, Gram-stain negative, non-sporulating, motile, rod-shaped bacterium designated strain NSA17 was isolated from leaf soil collected in Japan. This strain grew at 25 °C to 40 °C (optimum 35 °C) and pH 5.0 to 10.0 (optimum pH 6.5). The major cellular fatty acid and quinone system were C 16: 0 and ubiquinone 8. Phylogenetic analysis based on 16S rRNA gene sequences showed strain NSA17 to present as a member of the genus Bordetella . The draft genome of strain NSA17 consisted of 5,144,706 bp with a G + C content of 68.5% and comprising 5,740 predicted coding sequences. Multilocus sequence analysis based on partial sequences of four housekeeping genes ( atpD , gyrB , infB and rpoB ) showed that branching of strain NSA17 was distant to those of other Bordetella type strains. The genome average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain NSA17 and its closest related Bordetella type strains were less than 84.6%, 40.6% and 85.9%, respectively. Based on its phenotypic, chemotaxonomic, phylogenetic and phylogenomic features, strain NSA17 (= HUT-8147 T = KCTC 82065 T ) can be considered a novel species within the genus Bordetella with the proposed name Bordetella saccharivorans sp. nov.