Self-priming Dual-hairpin probe-mediated Primer Exchange Reaction for Label-free Fluorescent detection of MicroRNA in Alzheimer’s Disease

Key Points

• The aim is to develop a novel fluorescent biosensing platform for detecting microRNA biomarkers related to Alzheimer's disease.
• Developed a self-priming dual-hairpin probe system
• Employed a label-free detection method
• Integrated fluorescence for signal amplification
• Successfully detected specific microRNA associated with Alzheimer's disease
• Achieved high sensitivity in detection
• Demonstrated potential for early diagnosis and therapeutic monitoring