Chemoenzymatic synthesis of sulfated O-glycopeptides from human CD34

Key Points

• The research aims to explore the role of O-glycosylation in modulating the biofunction of CD34.
• Developed a chemoenzymatic strategy for synthesizing O-glycopeptides.
• Assembled a library of CD34 glycoforms efficiently.
• Performed biochemical analyses to assess modifications.
• Successfully synthesized various sulfated O-glycopeptides.
• Identified distinct biofunctional roles of different glycoforms of CD34.
• Demonstrated improved efficiency in glycopeptide assembly compared to traditional methods.