Blood is a key biological specimen in forensic analysis for both living and deceased individuals, playing a crucial role in drug testing, blood typing, DNA analysis, and bloodstain pattern examination. In forensics, the decomposition of blood holds particular importance because it is a major biological fluid in the human body and undergoes early chemical changes that attract insects and microorganisms to cadaveric sources. The odor signatures produced during the putrefactive process have recently gained forensic relevance, prompting studies to investigate volatile organic compounds (VOCs) from blood, tissues, animal proxies, and human cadavers to enhance human remains detection and recovery via technological or biological means. This study focuses on cadaveric blood odor profiling, evaluating VOC signatures from human cadavers in an anatomy laboratory using solid-phase micro-extraction (SPME) and gas chromatography–mass spectrometry (GC/MS) upon body receipt. A second phase entailed a degradation analysis using 7 human cadavers and a total of 28 postmortem samples repeatedly sampled over a 4-week period. The findings revealed an increasingly complex odor profile as decomposition progresses, with a notable rise in both the variety and concentration of VOCs. Room temperature samples exhibited a more diverse and rapid VOC release, while refrigerated samples showed slower degradation. These insights contribute to a deeper understanding of decomposition patterns and ultimately refine human remains detection methodologies.
Reddi et al. (Wed,) studied this question.