What question did this study set out to answer?

This research aims to uncover how miR-31 influences oocyte maturation through the MAPK8/JNK signaling pathway.

February 19, 2026

MiR-31 enhances porcine oocyte maturation via MAPK8/JNK-mediated cumulus expansion and cytoplasmic maturation

Key Points

This research aims to uncover how miR-31 influences oocyte maturation through the MAPK8/JNK signaling pathway.
Four treatment groups were used: miR-31 mimics, mimics negative control, miR-31 inhibition, and inhibition negative control.
Gene expression levels of PTGS2 and PTX3 were measured and compared across groups.
Oocyte quality was analyzed through mitochondrial membrane potential, ATP production, and redox homeostasis assessment.
MiR-31 overexpression significantly increased cumulus cell expansion and oocyte maturation rates.
Enhanced mitochondrial membrane potential and ATP production were observed with miR-31 overexpression.
Inhibition of miR-31 led to a higher cortical granule misdistribution rate and increased MAPK8/JNK pathway activity.

Abstract

Abstract This study elucidates the molecular mechanism by which porcine ovarian cumulus cell-derived miR-31 regulates oocyte IVM through the MAPK8/JNK signaling pathway, with particular emphasis on its biological roles in cumulus cell expansion and oocyte maturation quality. The experimental design comprised four treatment groups: miR-31 mimics group, mimics negative control (NC) group, miR-31 inhibition group, and inhibition NC group. The results showed: (1) miR-31 overexpression significantly enhanced cumulus cell expansion processes, upregulating PTGS2 (P 0.05) and PTX3 (P 0.01) gene expression, while concurrently increasing oocyte maturation rates (P 0.05). (2) Oocyte quality analysis revealed that miR-31 overexpression induced: Elevated mitochondrial membrane potential (P 0.05); Increased ATP production (P 0.05); Reduced lipid droplet size (P 0.05) with concurrent quantity increased (P 0.05); Improved redox homeostasis (P 0.05). Conversely, miR-31 inhibition demonstrated opposing effects: Elevated cortical granule misdistribution rate (P 0.05); Significant activation of MAPK8/JNK pathway activity (P 0.01). In conclusion, this study demonstrates that miR-31 coordinates cumulus cell expansion with oocyte metabolic reprogramming by targeting MAPK8, thereby enhancing nuclear-cytoplasmic maturation synchrony.

Perguntar à IA

Bookmark

Cite This Study

Zhang et al. (Mon,) studied this question.

synapsesocial.com/papers/6996a80aecb39a600b3ee55f https://doi.org/https://doi.org/10.1093/jas/skag036

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

Perguntar à IA

Bookmark